Process

- Denaturation – DNA is heated to 95°C which breaks the h bonds, strands seperate - Annealing - the temperature is decreased to 52°C so that primers can join to their complementary bases at the end of the DNA fragment - Elongation / Extension – the temperature is increased to 72°C, as this is the optimum temperature for Taq polymerase to build the complementary strands of DNA to produce the new identical double-stranded DNA molecules