Bio 102 Quiz #6 Review: Why are numerous replication cycles – each performed at three difference temperatures – a necessary part of a PCR reaction?

Question

Why are numerous replication cycles – each performed at three difference temperatures – a necessary part of a PCR reaction?

Incredible Snipe · Accepted Answer

The initial cycles do not produce many copies of the target sequence, but since the number of molecules produced doubles with each cycle, millions of copies are produced after 20-30 cycles.

Incredible Snipe · Answer

The initial cycles amplify DNA slowly, but as the temperature increases, DNA polymerase works more quickly to produce millions of copies of the target sequence.

Incredible Snipe · Answer

Once the enzyme starts working, it must continue until there are no remaining nucleotides.

Incredible Snipe · Answer

The bacteria used in the reaction divide with each cycle, replicating the target DNA.

Incredible Snipe · Answer

Only non-target DNA is made during the first 10 cycles, while only cycles 11-20 specifically target the gene of interest.

Bio 102 Quiz #6 Review

Why are numerous replication cycles – each performed at three difference temperatures – a necessary part of a PCR reaction?

The initial cycles do not produce many copies of the target sequence, but since the number of molecules produced doubles with each cycle, millions of copies are produced after 20-30 cycles.

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