GM process (2) producing DNA fragments

- Another method of producing DNA fragments is to use restriction enzymes - Named source of DNA (hair follicle...) - Digested/hydrolysed using restriction enzymes - Use a thermocycler - Heat to (95) to break h bonds - Cool to (55) allow primers to attach - Function of primers (allow polymerase to attach, locate specific seq) - Heat to (72) optimum temp for DNA polymerase - Free nucleotides bind with comp. nucleotides - Repeated many times