Neutral gene evolution test

Test if there is a significant deviation (NS) from neutral expectation. An allele may be associated with an extended area of linkage disequilibrium around it. We can compare interspecies and intraspecies variation. The tips and nodes of a tree should balance with the extent of allele sharing in the tree. Compare the ratio of synonymous to non-synonymous substitutions, under neutral conditions 20% of mutations should be synonymous