Lap score principle

 Alkaline phosphatase activity is present in varying degrees in the neutrophil and band form of the granulocytes The amount of dye precipitated is proportional to the amount of enzyme present. Generally, a high WBC with increased neutrophils is associated with increased enzyme. Relationship between the number of enzyme and the Neutrophil present is Directly proportional With the use of different substrate solution with the presence of Enzyme Alkaline Phosphatase in an alkaline ph, it will be hydrolyzed. Using working solution (ex. Fast Blue RR dye or Fast Blue BBN) it will result to an insoluble precipitate at the site of enzyme activity.