Principle of HDL Assay

The reaction between cholesterol other than HDL and the enzyme for cholesterol assay is suppressed by the electrostatic interaction between polyanions & cationic substances. • Hydrogen peroxide is formed by the free cholesterol in HDL by cholesterol oxidase. Oxidative condensation of EMSE and 4 AA is caused by hydrogen peroxide in the presence of peroxidase , and the absorbance of the resulting red purple quinone is measured to obtain the cholesterol value in HDL.