level: Level 3
Questions and Answers List
level questions: Level 3
| Question | Answer |
|---|---|
| ITS PRINCIPLE is Charged molecules or molecular fragments are generated in high-vacuum region, using a variety of methods for ION PRODUCTION. The ions are generated in the GAS PHASE so that they can then be manipulated by the application of either electric or magnetic fields to enable the DETERMINATION OF THEIR MOLECULAR WEIGHTS | MASS SPECTROMETRY |
| MASS SPECTROMETRY APPLICATION | 1 HIGHLY SPECIFIC METHOD for determining or confirming the IDENTITY or structure of drugs 2 Mass spectroscopy (MS) in conjunction w/ either gas chromatography (GC-MS) or liquid chromatography (LC-MS) provides a method for CHARACTERISING IMPURITIES in drugs |
| provide highly sensitive and specific methods for determining drugs and their metabolites | gas chromatography (GC-MS) and liquid chromatography (LC-MS) |
| MASS SPECTROMETRY Strengths: Limitations: | STRENGTHS 1 RAPID IDENTIFICATION of trace impurities, w/c should ideally be carried out using chromatographic separation in conjunction w/ mass spectrometry so that elemental composition can be determined 2 The METHOD OF CHOICE for MONITORING DRUGS and their metabolites in biological fluids because of its high sensitivity and selectivity 3 QC of therapeutic antibodies and peptides LIMITATIONS 1 MS is NOT CURRENTLY USED IN ROUTINE QC but is placed in Research and Development where it is used to solve specific problems arising from routine process or in-process development 2 Instrumentation is EXPENSIVE, requires highly trained personnel |