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level: Level 1 of Genetic Engineering

Questions and Answers List

level questions: Level 1 of Genetic Engineering

QuestionAnswer
How do bacteria contain genetic information?a small circular plasmid and a large circular chromosome
What is the chromosome and the plasmid made from?genes, each one coding for a different protein
What is genetic engineering?scientists can now remove genes from the plasmid and also insert specific genes from other organisms into it.
Stage 1 of genetic engineeringidentify the section of DNA that contains the required gene from the source chromosome
Stage 2 of genetic engineeringextract the required gene using a restriction endonuclease enzyme
Stage 3 of genetic engineeringinsert the required gene into a bacterial plasmid using a ligase enzyme, the plasmid is the vector (carrier of genetic info)
Stage 4 of genetic engineeringinsert the plasmid into a host cell (a bacterium)
Stage 5 of genetic engineeringculture the transformed cell to produce the required product/protein