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level: disha questions

Questions and Answers List

level questions: disha questions

QuestionAnswer
human genome project is closely associated with the rapid development of a new area in biology called asbioinformatics
genes of interest can be selected from a genomic library by usingDNA probes
what are palindromic base sequencepalindromic sequences in DNA molecule are group of bases that forms the same sequence when read in both forward and backward direction
plasmids are the extrachromosomal part in the bacteria and are useful inrecombinant DNA technology
plasmids are extra chromosomal material possesing single stranded DNA, they are present inin eukaryotes as well as in prokaryotes
in recombinant DNA technology, recombinants DNA are usualy transferred to E.coli, yeat beacuse of their rapid multiplicationthis technology is employed for combining DNA from two different organisms to produce recombinant DNA
restriction enzymes cut the strand of DNA to produce sticky ends.(named so because they form h bond with theiir complemetary counter parts)the stickness of the ends facilitates the action of the enzyme DNA ligase, not DNA polymerase.
the DNA fragments separated on an agarose gel can be visualised after staining withethidium bromide
a gene whose expression helps to identify transformed cell is known asselectable marker
some features of plasmidsindependent replication, circular structure, transferable
the taq polymerase enzyme is obtained fromthermus aquaticus
the dna molecule to which the gene of interest is integrated for cloning is calledvector
an analysis of chromosomal DNA using the southern hybridisation technique useselectrophoresis, blotting and autoradiology
during the proccess of isolation of DNA chilled ethanol is added toprecipitate DNA
the colonies of recombinant bacteria appears white in contrast to blue colonies of non-recombinant bacteria because ofnon-recombinant bacteria containing beta-galactosidase
genes of interest can be selected from a genomic library by usingDNA probes
stirred tank bioreactors hav been designed foravailability of oxygen throughout the process
the linking of antibiotic resistance gene with the plamid vector became possible withDNA ligase
chromosomes in a bacterial cell can be 1-3 in number andare always circular
the plasmids present in bacterial cells arecircular double helical DNA molecules
in vitro clonal propagation in plants is characterised byPCR and RAPD
PCR and restriction fragment length polymorphism are the methods forgenetic fingerprinting
at what temp. does the DNA polymerase used in PCR remain activeat high temperature
for transformation, micro-particles coated with DNA to be bombarded with gene gun are made up ofgold or tungsten
biolistics(gene gun) is suitable forconstructing recombinant DNA by joining with vectors