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level: aiims questions

Questions and Answers List

level questions: aiims questions

QuestionAnswer
restriction endonucleases are used asmolecular knives for cutting DNA at specific sites
c-DNA probes are copied from the messenger RNA molecules with the help ofreverse transcriptase
in this procedure transient pores are made in the cell membrane to introduce gene constructselectroporation
the first step in the southern blot techniquedigestion of DNA by restriction enzymes
the polymerase chain reaction technology was discovered bykarry mullis in 1984
after 4 PCR cycles how many DNA molecules are formed from one DNA template moleculesfrom a single DNA template molecules, it is possible to generate 2^n DNA molecules after n no. of cycles in polymerase chain reaction
why are plasmids used as vectors for inserting foreign DNA into a cell?because they can carry a foreign DNA fragment when inserted into it .
which organism has found extensive use in genetic engineeringagrobacterium tumefaciens
these are endonucleases which cleave DNA at specific sites and these are produced by bacteria for their defense mechanism.restriction enzymes
these are generated by inducing a foreign DNA into a cell and regenerating a plant from that celltransgenic plants
which vector is used for cloning in higher organismsretrovirus
(biochemistry) a long linear polymer found in the nucleus of a cell and formed from nucleotides and shaped like a double helix; associated with the transmission of genetic informationDNA polymerase
if a recombinant DNA bearing gene for ampicillin resistance if transferred into E. coli cells and the host cells are transferred onto the agar plates containing ampicillin what will happen.the transformed receipient cell will grow and the non transformed cell would die
in the PCR technology the DNA is segment is replicated over a billion times. this is catalyzed why which enzymeTaq polymerase
DNA polymerase remain active in which temperaturehigh temperature
cohen and boyer isolated an antibiotic resistance gene in which year1972
restriction enzymes ECO RI cuts the DNA between bases G and A only when the sequence in DNA isGAATTC
the mobile genetic element istransporon
GAATTC is the recognition site for which restriction endonucleaseEco RI I
which bioengineered bacteria is utilized for cleaning of marine oil slicks?pseudomonas putida
at the scene of the crime. bloodstains are obtained, which part of blood is used for DNA profiling.leucocytes
what is used in DNA recombinant techniquevirus
the correct order of the process in PCR isdenaturation, annealing and extenstion